| ORIGINAL ARTICLE |
|
| Year : 2016 | Volume
: 34
| Issue : 3 | Page : 273-279 |
Evaluation of chemokines in gingival crevicular fluid in children with dental caries and stainless steel crowns: A clinico-biochemical study
Naveen Kommineni Kumar, Veera Kishore Kasa Reddy, Prathyusha Padakandla, Harshini Togaru, Swathi Kalagatla, Sarath N Chandra
Department of Pedodontics, C.K.S. Teja Institute of Dental Sciences, Tirupati, Andhra Pradesh, India
Correspondence Address:
Naveen Kommineni Kumar
Department of Pedodontics, C.K.S. Teja Institute of Dental Sciences, Tirupati - 517 507, Andhra Pradesh
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0970-4388.186754
Aims and Objectives: The study was conducted to detect the presence of macrophage inflammatory protein-1α (MIP-1α) and MIP-1β and estimate their levels in gingival crevicular fluid (GCF) in children with dental caries and stainless steel crowns. Materials and Methods: A total of 80 children with primary dentition were selected and categorized into four groups with twenty in each group; Group 1 - healthy subjects, Group 2 - dental caries, Group 3 - dental caries involving the pulp, and Group 4 - stainless steel crowns. GCF samples were collected by an extra-crevicular method with microcapillary pipettes. The GCF samples were quantified by ELISA and the levels of MIP-1α and MIP-1β were determined. Results: MIP-1α and MIP-1β were detected in all the samples. Highest mean concentration in GCF was obtained for Group 3 followed by Groups 2 and 4 while the lowest concentration was seen in Group 1. This suggests that MIP-1α and MIP-1β levels in GCF increased proportionately with the inflammation. Conclusions: GCF serves as a noninvasive diagnostic fluid to measure biomarkers released during dental caries initiation and progression. MIP-1α and MIP-1β chemokines can be considered as novel biomarkers, in biological mechanism underlying the pathogenesis and inflammation in children with dental caries and stainless steel crowns.
[FULL TEXT] [PDF]*
|
