Journal of Indian Society of Pedodontics and Preventive Dentistry
Journal of Indian Society of Pedodontics and Preventive Dentistry
                                                   Official journal of the Indian Society of Pedodontics and Preventive Dentistry                           
Year : 2020  |  Volume : 38  |  Issue : 4  |  Page : 350--354

In vitro evaluation of milk-based, soy-based, and amino acid-based infant formulas on Streptococcus mutans biofilm formation


Hira Sadan, BM Shanthala, MA Zareena, George Babu, Vidhya Vijayan 
 Department of Pediatric and Preventive Dentistry, Coorg Institute of Dental Sciences, Kudagu, Karnataka, India

Correspondence Address:
Dr. Hira Sadan
Department of Pediatric and Preventive Dentistry, Coorg Institute of Dental Sciences, K.K. Campus, Maggula Village, Virajpet, Kudagu - 571 218, Karnataka
India

Background and Objective: Infant formulas are based on milk, and the addition of simple carbohydrates as a caloric source, for infants. The carbohydrates added in infant formulas can cause a significant increase of Streptococcus mutans in the oral cavity of infants adding to their cariogenicity. Aim: The aim of the study was to assess and compare the biofilm formation in three commercially available infant formulas;which are based on milk, soy and amino acid. Settings and Design: In vitro microbiological assay of Streptococcus mutans biofilm formation in milk based, soy based and amino acid based infant formulas. Materials and Methods: Twenty-four hour-cultured S. mutans and microtiter plates were used for analysis. At microtiter plate, 190 μL of modified TSB broth containing SBF, MBF, amino acid-based infant formulas, and dairy whitener as a positive control in five dilutions (1:05, 1:10, 1:20, 1:40, and 1:80) was added into respective wells. 10 μL of cultured S. mutans was inoculated into the wells and incubated at 37°C for 24 h. Biofilm was washed, fixed, and stained with crystal violet. The absorbance was measured to evaluate biofilm growth, which was read as optical densities in a spectrophotometer at 490 nm and was tabulated. Results: Three infant formulas tested showed S. mutans biofilm growth. Minimal biofilm growth was observed in amino acid-based formula at 1:80 dilution, followed by MBF at 1:10 dilution and SBF at 1:80 dilution. Conclusion: Commercially available infant formulas favor S. mutans biofilm growth and can be cariogenic. Amino acid-based infant formula was found to have less S. mutans biofilm growth than MBF and SBF.


How to cite this article:
Sadan H, Shanthala B M, Zareena M A, Babu G, Vijayan V. In vitro evaluation of milk-based, soy-based, and amino acid-based infant formulas on Streptococcus mutans biofilm formation.J Indian Soc Pedod Prev Dent 2020;38:350-354


How to cite this URL:
Sadan H, Shanthala B M, Zareena M A, Babu G, Vijayan V. In vitro evaluation of milk-based, soy-based, and amino acid-based infant formulas on Streptococcus mutans biofilm formation. J Indian Soc Pedod Prev Dent [serial online] 2020 [cited 2021 May 12 ];38:350-354
Available from: https://www.jisppd.com/article.asp?issn=0970-4388;year=2020;volume=38;issue=4;spage=350;epage=354;aulast=Sadan;type=0